miRNA-146a and miRNA-202-3p Attenuate Inflammatory Response by Inhibiting TLR4, IRAK1, and TRAF6 Expressions in Rats following Spinal Cord Injury

Spinal cord injury (SCI) is a catastrophic disease that induces complex cascade of cellular reactions at the local lesion area, including secondary cell death and inflammatory reactions. Accumulating evidence has showed pro- anti-inflammatory roles microRNAs (miRNAs), class small RNAs, in SCI. The present study aimed investigating effects two miRNAs, miRNA-146a miRNA-202-3p, on response after Initially, we found expression levels miRNA-202-3p were increased plasma samples 32 SCI patients days 3 7 admission rat spinal modeling compared with healthy controls sham-operated rats, respectively. TLR4, IRAK1, TRAF6 declined 1, 3, rats. Injection mimic or decreased expressions modeling, while injection antagomir produced opposed results. Subsequent results tumor necrosis factor-α (TNF-α), IL-1β, IL-6, IL-8 upregulated serum TNF-α, yielded higher supernatants PC12 cells transfected anti-miRNA-146a anti-miRNA-202-3p than those si-TLR4, si-IRAK1, si-TRAF6. These findings support notion miRNA-146a/miRNA-202-3p exerts functions